In the field of cancer drug discovery, 3D cell culture is a “must” in order to obtain more physiologically relevant information and more predictive data for “in vivo” tests.

The benefits of 3D vs 2D can be summarized in a single quote: “3D cell culture assays provide more physiologically relevant information and more predictive data for “in vivo” tests. 2D cell culture assays do not mimic adequately the tumor architecture “in vivo” due to the abnormal morphology that is adopted by the cells in monolayer cultures.

Counting on 3D tools like Cell2Sphere, definitely you do not need to make any changes in your lab. Conventional cell culture material and reagents is all what you need.

If you use 2D or fresh 3D you just need to follow the instructions in the Cell2Sphere kit. No implementation needed.

Cell2Sphere saves you significant time of daily lab work that you can dedicate to planning experiments.

To obtain false hits from the tested compounds against tumor cells.

Cell2Sphere is a ready-to-use all-in-one scaffold-free 3D tumor cell culture kit. The Cell2Sphere kit consists of a plate with the spheroid seeds and a bottle of culture medium. It is stored frozen at -80ºC.

Co-culture // Migration assay // Flow cytometry // Immunofluorescence, Immunohistochemistry // Secretome analysis // Gen/protein expression analysis // Gen reporter activity // Protein activity

The Cell2Sphere YOUROWN format allows you to ask for personalized plates with your own cell lines

No, because the media provided with the Cell2Sphere kit is specific for 3D cell culture with a different composition than standard culture medium

The Cell2Sphere culture medium allows the spheroids to grow in optimal conditions, fact that affects directly to the results obtained in the assays performed