세포.조직 기반 연구
세포.조직 기반 연구

FAQ's : The Cell2Sphere™ Lorem ipsum dolor sit amet

FAQ’S

The Cell2Sphere™ Lorem ipsum dolor sit amet
Is 3D Really an option for my research objectives?

In the field of cancer drug discovery, 3D cell culture is a “must” in order to obtain more physiologically relevant information and more predictive data for “in vivo” tests.

What are the benefits of 3D assays compared to 2D?

The benefits of 3D vs 2D can be summarized in a single quote: “3D cell culture assays provide more physiologically relevant information and more predictive data for “in vivo” tests. 2D cell culture assays do not mimic adequately the tumor architecture “in vivo” due to the abnormal morphology that is adopted by the cells in monolayer cultures.

Do I need to make changes in my lab?

Counting on 3D tools like Cell2Sphere, definitely you do not need to make any changes in your lab. Conventional cell culture material and reagents is all what you need.

What is needed to implement this solution if I use now 2D or fresh 3D?

If you use 2D or fresh 3D you just need to follow the instructions in the Cell2Sphere kit. No implementation needed.

How does it affect the daily work in my lab?

Cell2Sphere saves you significant time of daily lab work that you can dedicate to planning experiments.

What are the risks of not using this innovative solution?

To obtain false hits from the tested compounds against tumor cells.

What does it consist of?

Cell2Sphere is a ready-to-use all-in-one scaffold-free 3D tumor cell culture kit. The Cell2Sphere kit consists of a plate with the spheroid seeds and a bottle of culture medium. It is stored frozen at -80ºC.

What other tests can I run with this technology?

Co-culture // Migration assay // Flow cytometry // Immunofluorescence, Immunohistochemistry // Secretome analysis // Gen/protein expression analysis // Gen reporter activity // Protein activity

Are we limited to the available cell lines? Or can we use our own?

The Cell2Sphere YOUROWN format allows you to ask for personalized plates with your own cell lines

Can I use a standard culture medium?

No, because the media provided with the Cell2Sphere kit is specific for 3D cell culture with a different composition than standard culture medium

What are the benefits of using the specific culture medium?

The Cell2Sphere culture medium allows the spheroids to grow in optimal conditions, fact that affects directly to the results obtained in the assays performed