Hydrophobic Interaction Chromatography (HIC) Media 제품 문의
HIC is a versatile technique and can show high selectivity to individual molecules according to their exposed hydrophobic zones. It is particularly useful for intermediate and final-stage purifications.
Butyl SepFast™, Phenyl SepFast™, and Octyl SepFast™ are hydrophobic interaction chromatography (HIC) adsorbents with carbon chains of C4, C6 (benzene ring), and C8, respectively. They are specially designed for the purification of biological molecules based on their hydrophobicity profiles.
HIC media requires much milder purification conditions than reversed phase chromatography (RPC) media. Hence biological activity can be maintained in HIC separations unlike RPC operations.
HIC media normally binds at moderate to high salt concentrations. It is therefore logical to place a HIC step after an IEX step where molecules are usually eluted at high salt conditions.
SepFast™ HIC media offers a broad range of choices from C4 to C8. Generally speaking, the longer the carbon chain, the higher the surface hydrophobicity.
Biotoolomics HIC media gives you:
- High sample loading capacity
- High separation power
- Available at all pack sizes from analytical up to full manufacturing scale.
- Chemically stable Agarose base matrix.
- Can be produced in high and low ligand densities, alternate crosslinking levels and different bead sizes to optimise the media to the separation.
- All versions can be scaled up and produced for GMP use
- More choices than other suppliers.
The feature and selection guide is listed as follows:
4HF serial
(50µm - 150µm) | 6HF serial
(50µm - 150µm) | Large Beads
(150µm - 350µm) | HighRes
(25µm - 50µm) |
| The above HIC media is designed to purify most medium to large proteins | The above HIC media is designed to purify peptides or smaller proteins | The above HIC media is designed to purify proteins from crude or viscous samples | The above HIC media is designed to purify proteins
that require high resolution |
Characteristics of SepFast HIC Media
| Ligand | Butyl | Phenyl | Octyl |
| Matrix | Highly cross-linked agarose; 4% agarose for SepFast 4HF, 6% agarose for SepFast 6HF |
Functional
group | -(CH2)3CH3 | -C6H6 | -(CH2)7CH3 |
Ligand
density | Around 40 µmol/ml | Around 25 µmol/ml;
Around 40 µmol/ml (high sub) | Around 4 µmol/ml |
Binding
capacity | >20 mg lyzosyme / ml | >20 mg BSA / ml | >20 mg BSA / ml |
Particle
size | 25 - 50 µm (HighRes), 50 - 150 µm (4HF and 6HF), 150 - 350 µm (Large Beads) |
Pressure
-flow
property* | >100 cm/h for HighRes; >300 cm/h for 4HF serials; >500 cm/h for 6HF serials; >1500 cm/h for Large Beads |
Operational
pressure | Up to 3 bar |
pH
stability | 2-14 (short term) and 3-12 (long term) |
Working
temperature | 4oC – 30oC |
Chemical
stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% acetonitrile, 30% isopropanol, 70% ethanol, 2 M (NH4)2SO4 |
| Storage | 20% ethanol |
*Measured in a 32 mm ID column at a bed height of 15 cm.
Ordering information
| Description | Quantity | Code no. | Price GBP |
| HIC SepFast Selection Kit | 7 x 1 ml column packed with Butyl 4HF, Butyl 6HF, Phenyl 4HF, Phenyl 6HF, Phenyl 6HF high sub, Octyl 4HF and Octyl 6HF, respectively | 480000-7x1ML | £183.75 |
SepFast Butyl Products | SepFast Phenyl Products | SepFast Octyl Products |
| Description | Quantity | Code no. | Price GBP |
| Butyl SepFast 4HF | 25 ml | 480101-25ML | £99.67 |
| | 100 ml | 480101-100ML | £262.51 |
| | 1 Litre | 480101-1L | POR |
| Butyl SepFast 4HF prepacked column | 5 x 1 ml | 480101-5x1ML | £135.45 |
| | 5 x 5 ml | 480101-5x5ML | £281.40 |
| Butyl SepFast 6HF | 25 ml | 480301-25ML | £99.67 |
| | 100 ml | 480301-100ML | £262.51 |
| | 1 Litre | 480301-1L | POR |
| Butyl SepFast 6HF prepacked column | 5 x 1 ml | 480301-5x1ML | £135.45 |
| | 5 x 5 ml | 480301-5x5ML | £281.40 |
| Butyl SepFast Large Beads | 25 ml | 480201-25ML | £99.67 |
| | 100 ml | 480201-100ML | £262.51 |
| | 1 Litre | 480201-1L | POR |
| SepFast Butyl Products | SepFast Phenyl Products | SepFast Octyl Products |
| Description | Quantity | Code no. | Price GBP |
| Phenyl SepFast 4HF | 25 ml | 481001-25ML | £99.67 |
| | 100 ml | 481001-100ML | £262.51 |
| | 1 Litre | 481001-1L | POR |
| Phenyl SepFast 4HF prepacked column | 5 x 1 ml | 481001-5x1ML | £135.45 |
| | 5 x 5 ml | 481001-5x5ML | £281.40 |
| Phenyl SepFast 6HF | 25 ml | 481201-25ML | £99.67 |
| | 100 ml | 481201-100ML | £262.51 |
| | 1 Litre | 481201-1L | POR |
| Phenyl SepFast 6HF prepacked column | 5 x 1 ml | 481201-5x1ML | £135.45 |
| | 5 x 5 ml | 481201-5x5ML | £281.40 |
| Phenyl SepFast 6HF High sub | 25 ml | 481202-25ML | £99.67 |
| | 100 ml | 481202-100ML | £262.51 |
| | 1 Litre | 481202-1L | POR |
| Phenyl SepFast 6HF High sub prepacked column | 5 x 1 ml | 481202-5x1ML | £135.45 |
| | 5 x 5 ml | 481202-5x5ML | £281.40 |
| Phenyl SepFast HighRes | 25 ml | 481101-25ML | £120.75 |
| | 100 ml | 481101-100ML | £315.00 |
| | 1 Litre | 481101-1L | POR |
| Phenyl SepFast HighRes prepacked column | 5 x 1 ml | 481101-5x1ML | £161.70 |
| | 5 x 5 ml | 481101-5x5ML | £308.70 |
| Phenyl SepFast Large Beads | 25 ml | 481301-25ML | £99.67 |
| | 100 ml | 481301-100ML | £262.51 |
| | 1 Litre | 481301-1L | POR |
| SepFast Butyl Products | SepFast Phenyl Products | SepFast OctylProducts |
| Description | Quantity | Code no. | Price GBP |
| Octyl SepFast 4HF | 25 ml | 481601-25ML | £99.67 |
| | 100 ml | 481601-100ML | £262.51 |
| | 1 Litre | 481601-1L | POR |
| Octyl SepFast 4HF prepacked column | 5 x 1 ml | 481601-5x1ML | £135.45 |
| | 5 x 5 ml | 481601-5x5ML | £281.40 |
| Octyl SepFast 6HF | 25 ml | 481801-25ML | £99.67 |
| | 100 ml | 481801-100ML | £262.51 |
| | 1 Litre | 481801-1L | POR |
| Octyl SepFast 6HF prepacked column | 5 x 1 ml | 481801-5x1ML | £135.45 |
| | 5 x 5 ml | 481801-5x5ML | £281.40 |
| Octyl SepFast Large Beads | 25 ml | 481701-25ML | £99.67 |
| | 100 ml | 481701-100ML | £262.51 |
| | 1 Litre | 481701-1L | POR |
Note: HIC media with lower or higher ligand density can be custom-made to increase recovery or binding capacity. Please contact us for more information.
Hydrophobic Interaction Chromatography (HIC) Media 제품 문의
HIC is a versatile technique and can show high selectivity to individual molecules according to their exposed hydrophobic zones. It is particularly useful for intermediate and final-stage purifications.
Butyl SepFast™, Phenyl SepFast™, and Octyl SepFast™ are hydrophobic interaction chromatography (HIC) adsorbents with carbon chains of C4, C6 (benzene ring), and C8, respectively. They are specially designed for the purification of biological molecules based on their hydrophobicity profiles.
HIC media requires much milder purification conditions than reversed phase chromatography (RPC) media. Hence biological activity can be maintained in HIC separations unlike RPC operations.
HIC media normally binds at moderate to high salt concentrations. It is therefore logical to place a HIC step after an IEX step where molecules are usually eluted at high salt conditions.
SepFast™ HIC media offers a broad range of choices from C4 to C8. Generally speaking, the longer the carbon chain, the higher the surface hydrophobicity.
Biotoolomics HIC media gives you:
The feature and selection guide is listed as follows:
(50µm - 150µm)
(50µm - 150µm)
(150µm - 350µm)
(25µm - 50µm)
that require high resolution
Characteristics of SepFast HIC Media
group
density
Around 40 µmol/ml (high sub)
capacity
size
-flow
property*
pressure
stability
temperature
stability
*Measured in a 32 mm ID column at a bed height of 15 cm.
Ordering information
SepFast Butyl Products
SepFast Phenyl Products
SepFast OctylProducts
Note: HIC media with lower or higher ligand density can be custom-made to increase recovery or binding capacity. Please contact us for more information.