SepFastTM Gel Filtration Media 제품 문의
Introduction
SepFast gel filtration media is specially designed for high resolution and high recovery fractionation of biological molecules based on their molecular weights. Gel filtration (or called size exclusion chromatography) is a proven technique, which is widely used for size-based molecular separations.
SepFast gel filtration media has a balanced design to offer high recovery and high selectivity according to individual applications. The core advantages are:
- High resolution
- High selectivity
- High recovery
- Highly scalable
- Excellent physical and mechanical stability
- Cost effective
The base matrix is made of agarose or a composite of polysaccharides that have been highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.
The selection guide is listed as follows:
| | SepFast GF-HS |
| | SepFast GF-HS-M | SepFast GF-HS-L |
| Separation range | 3x103 - 7x104 | 6x103 - 6x105 |
| Application | Preparative grade separation for research, process development and large-scale productions |
| Particle Size(µm) | 20-50 | 20-50 |
| Format supplied | Loose resin and pre-packed columns |
References Featuring Biotoolomics SepFast GF-HS-L Products:
de Cassan, S.C., Shakri, A.R., Llewellyn, D., Elias, S.C., Cho, J.S., Goodman, A.L., Jin, J., Douglas, A.D., Suwanarusk, R., Nosten, F.H. and Rénia, L., 2015. Preclinical assessment of viral vectored and protein vaccines targeting the Duffy-binding protein region II of Plasmodium vivax. Frontiers in immunology, 6, p.348
Jin, J., Tarrant, R.D., Bolam, E.J., Angell-Manning, P., Soegaard, M., Pattinson, D.J., Dulal, P., Silk, S.E., Marshall, J.M., Dabbs, R.A. and Nugent, F.L., 2018. Production, quality control, stability, and potency of cGMP-produced Plasmodium falciparum RH5. 1 protein vaccine expressed in Drosophila S2 cells. NPJ vaccines, 3(1), p.32
Characteristics of Gel Filtration Media
| Matrix | Highly cross-linked agarose and dextran composites |
| Particle size | Around 35 µm (in the range of 20 – 50 µm) |
| Operational pressure | 3 bar (0.3 MPa, 42 psi) for SepFast GF-HS-L
4 bar (0.4 Mpa, 56 psi) for SepFast GF-HS-M |
| Operating flow velocity | Normally 10-50 cm/hour |
| pH stability | 2-14 (short term) and 3-12 (long term) |
Working
temperature | 4oC – 30oC |
| Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol |
| Avoid | Oxidizing agents |
| Storage | The media should be stored in 20% ethanol to prevent microbial growth. Store the media at a temperature of +2oC to +8oC. |
Ordering information
| Description | Quantity | Code no. | Price GBP |
SepFast GF-HS-M
(SepFast SEC 3-70KDa prep grade) | 25 ml | 540201-25ML | £120.39 |
| | 150 ml | 540201-150ML | £436.49 |
| | 1 Litre | 540201-1L | POR |
| | We also provide other bulk volumes. Please contact us for further information. |
| SepFast GF-HS-M column | 16 x 600 mm | 3-70-16x600 | £1383.42 |
| | 26 x 600 mm | 3-70-26x600 | POR |
| | We also provide pre-packed columns with other diameters and heights. Please contact us for further information |
SepFast GF-HS-L
(SepFast SEC 6-600KDa prep grade) | 25 ml | 540101-25ML | £120.39 |
| | 150 ml | 540101-150ML | £436.49 |
| | 1 Litre | 540101-1L | POR |
| | We also provide other bulk volumes. Please contact us for further information. |
| SepFast GF-HS-L column | 16 x 600 mm | 6-600-16x600 | £1383.42 |
| | 26 x 600 mm | 6-600-26x600 | POR |
| | We also provide pre-packed columns with other diameters and heights. Please contact us for further information |
SepFastTM Gel Filtration Media 제품 문의
Introduction
SepFast gel filtration media is specially designed for high resolution and high recovery fractionation of biological molecules based on their molecular weights. Gel filtration (or called size exclusion chromatography) is a proven technique, which is widely used for size-based molecular separations.
SepFast gel filtration media has a balanced design to offer high recovery and high selectivity according to individual applications. The core advantages are:
The base matrix is made of agarose or a composite of polysaccharides that have been highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.
The selection guide is listed as follows:
References Featuring Biotoolomics SepFast GF-HS-L Products:
de Cassan, S.C., Shakri, A.R., Llewellyn, D., Elias, S.C., Cho, J.S., Goodman, A.L., Jin, J., Douglas, A.D., Suwanarusk, R., Nosten, F.H. and Rénia, L., 2015. Preclinical assessment of viral vectored and protein vaccines targeting the Duffy-binding protein region II of Plasmodium vivax. Frontiers in immunology, 6, p.348
Jin, J., Tarrant, R.D., Bolam, E.J., Angell-Manning, P., Soegaard, M., Pattinson, D.J., Dulal, P., Silk, S.E., Marshall, J.M., Dabbs, R.A. and Nugent, F.L., 2018. Production, quality control, stability, and potency of cGMP-produced Plasmodium falciparum RH5. 1 protein vaccine expressed in Drosophila S2 cells. NPJ vaccines, 3(1), p.32
Characteristics of Gel Filtration Media
4 bar (0.4 Mpa, 56 psi) for SepFast GF-HS-M
temperature
Ordering information
(SepFast SEC 3-70KDa prep grade)
(SepFast SEC 6-600KDa prep grade)