단백질 기반 연구

Functioning similarly to an old-fashioned darkroom photograph, the image of the protein sample is captured using a high-powered x-ray machine. The procedure has a high sensitivity rate and the finished image has no optical signal loss.While the method is still preferred in countries like the US, the use of x-ray film in western blotting has its share of downsides:

Utilizing industrial-grade SLR cameras to capture the required images, the cooled CCD western blot method is making itself known in the field as an alternative to x-ray film. Compared to the traditional x-ray film western blot method, the cooled CCD process boasts accurate quantitative imaging, a wider range of images, and digital documentation, among others.

Despite its set of advantages, the cooled CCD western blot imaging system has its own set of setbacks, which include:

Contact chemiluminescence imaging is an imaging process in which the lens is removed, allowing the sample to direct contact with the photographic chip so the imaging process can be done in just 1 second. Moreover, the sensor chip area is 130 times larger than the conventional cold CCD imaging, and the individual pixel size area is also increased by over 400 times.

This innovative design eliminates many bottlenecks of traditional cold CCD imaging:

• The western film is directly attached to the photographic chip for imaging, all signals are captured without any light loss, which greatly improves the sensitivity and speed of imaging;

• The super large size of the photographic chip with ultra high signal acquisition capacity improves the sensitivity and speed of imaging;

• The huge pixels can increase the quantification range by two orders of magnitude, enabling precise quantification of samples;

• The new imaging technology removes any lens for imaging, thus eliminates image distortion caused by the lens and provides more accurate results.