SpheroSeev can help!
Improve your spheroid, organoid or co-culture model using our spider silk biomimicry technology
SpheroSeev is a recombinant bio-polymer fiber with the strength and elasticity of spider silk, providing mechanical support and ECM-like environment for organoid formation in a consistent and reproducible manner.
Better Viability, Longevity and Functionality
|Confocal image of a spheroid containing SpheroSeev fibers (green) with cells (blue).||Confocal image of a stained SpheroSeev fibers.|
|Spheroid created with primary hepatocytes (left) compared with larger spheroid created with hepatocytes and SpheroSeev (right).|
|SpheroSeev enables cells to maintain their natural shape and interact with each other in a physiological manner, improving their viability and functionality in the spheroid format||SpheroSeev fibers have high porosity, enabling better access to oxygen and nutrients, thereby delaying the formation of a necrotic core|
|Adipose-derived mesenchymal stem cells (MSCs) cultured as spheroid with SpheroSeev demonstrate tissue-like spread morphology and higher viability than spheroid without SpheroSeev. [Green – viable cells, Red – dead cells]||L929 cell line cultured as spheroid with SpheroSeev demonstrates delayed necrotic core formation and higher viability.|
[Stained with PI and CalceinAM] [Green – viable cells, Red – dead cells]
|Superior viability||Better longevity||Exceptional functionality|
|ATP quantification test on primary rat hepatocytes shows that after 40 days spheroids with SpheroSeev have the same viability levels as the control spheroids in their first two weeks of culture.||SpheroSeev increases rat hepatocyte spheroid longevity for over 41 days compared with non-SpheroSeev spheroids, which disintegrate after ~20 days.||Albumin secretion test on primary rat hepatocytes shows that even after 20 days the spheroid with SpheroSeev retains good functionality while the control tissue shows no activity at all.|
|Increased viability of DU145 cancer cell line||SpheroSeev maintains stem cell spheroid differentiation||Better functionality with SpheroSeev|
|ATP quantification test on DU145 spheroids show that ATP level is higher when SpheroSeev concentration is greater.||hMSCs spheroids with and without SpheroSeev were cultured in adipogenic/osteogenic differentiation medium for 19 days|
[Stained with oil red and alizarin red respectively]
|SpheroSeev rat hepatocyte spheroids have higher basal and induced activity compared with control spheroids.|
Use SpheroSeev to better mimic natural morphology and behavior of cells, increase the productivity of your research, and reduce overall study costs and time