SpheroSeev : Having problems with your 3D cell culture?

2021-05-03
조회수 308

주문 및 샘플신청 seevix


Try out SpheroSeev with almost no effort!

Get a sample (shipping fee only)
Add the protein fiber to an existing experiment (a 5-minute protocol)
Discover
 the benefits of SpheroSeev: within days your organoid will be bigger and more vital and will survive longer


SpheroSeev can help!

Improve your spheroid, organoid or co-culture model using our spider silk biomimicry technology

SpheroSeev is a recombinant bio-polymer fiber with the strength and elasticity of spider silk, providing mechanical support and ECM-like environment for organoid formation in a consistent and reproducible manner.

Better Viability, Longevity and Functionality

Confocal image of a spheroid containing SpheroSeev fibers (green) with cells (blue).Confocal image of a stained SpheroSeev fibers.

Improve your chances of successful research:

SpheroSeev combines the advantages of scaffold-based and scaffold-free technologies.

SpheroSeev fibers’ nano and micro dimensions are similar to the natural ECM, providing a tissue-like environment for cells.

SpheroSeev fibers have high porosity, enabling better access to oxygen and nutrients, thereby supporting prolonged viability and functionality.

SpheroSeev fibers have extra strength and elasticity, providing mechanical cushioning and protection to individual cells within the organoid.


Applications

Drug discovery      Stem cells research      Cancer research      Tissue engineering   3D bioprinting



Results in just a few days!



SpheroSeev’s proven benefits for your cell culture research:



SpheroSeev fibers’ nano and micro dimensions are similar to the natural ECM, providing a tissue-like environment for cells



Spheroid created with primary hepatocytes (left) compared with larger spheroid created with hepatocytes and SpheroSeev (right).
  1. Confocal image of a stained SpheroSeev fibers
  2. TEM image of microfiber comprised of 10 nm nanofibers (insert)


SpheroSeev enables cells to maintain their natural shape and interact with each other in a physiological manner, improving their viability and functionality in the spheroid formatSpheroSeev fibers have high porosity, enabling better access to oxygen and nutrients, thereby delaying the formation of a necrotic core
Adipose-derived mesenchymal stem cells (MSCs) cultured as spheroid with SpheroSeev demonstrate tissue-like spread morphology and higher viability than spheroid without SpheroSeev. [Green – viable cells, Red – dead cells]L929 cell line cultured as spheroid with SpheroSeev demonstrates delayed necrotic core formation and higher viability.
[Stained with PI and CalceinAM] [Green – viable cells, Red – dead cells]


Superior viabilityBetter longevityExceptional functionality
ATP quantification test on primary rat hepatocytes shows that after 40 days spheroids with SpheroSeev have the same viability levels as the control spheroids in their first two weeks of culture.SpheroSeev increases rat hepatocyte spheroid longevity for over 41 days compared with non-SpheroSeev spheroids, which disintegrate after ~20 days.Albumin secretion test on primary rat hepatocytes shows that even after 20 days the spheroid with SpheroSeev retains good functionality while the control tissue shows no activity at all.
Increased viability of DU145 cancer cell lineSpheroSeev maintains stem cell spheroid differentiationBetter functionality with SpheroSeev
ATP quantification test on DU145 spheroids show that ATP level is higher when SpheroSeev concentration is greater.hMSCs spheroids with and without SpheroSeev were cultured in adipogenic/osteogenic differentiation medium for 19 days
[Stained with oil red and alizarin red respectively]
SpheroSeev rat hepatocyte spheroids have higher basal and induced activity compared with control spheroids.


Use SpheroSeev to better mimic natural morphology and behavior of cells, increase the productivity of your research, and reduce overall study costs and time


Here’s more of why to use SpheroSeev in your research:

  • Easy to use (a 5-minute process)
  • Long shelf life and sterile (up to 1 year)
  • Maximum flexibility to manipulate the 3D cell culture environment for different needs
  • High consistency for reproducible cell-based assays
  • Minimal protocol adaption to switch from 2D or currently used 3D
  • Compatible with automated handling and imaging equipment
  • Does not degrade or undergo alteration over the course of an experimen


Customers Reviews



I used “SpheroSeev” for primary glioblastoma cell culture. It helps to form nice and stable tumorspheres, which I never achieved before. With other kinds of matrix, my GBM cell always tends to differentiate, but “SpheroSeev” keeps the stemness of the cells. Great product!

Xue Sun
Ph.D. Student, Oren Ram Lab
Hebrew University

Human normal primary spheroids production using “SpheroSeev”, dramatically increased viability.

Orit Harari-Steinberg, Ph.D, Senior
Researcher & Lab Manager
Regenerative Medicine Research Lab
Pediatric Stem Cell Research Institute
Sheba Medical Center

“SpheroSeev” 3D matrix really works well. I could find a nice spheroid. Product was used with colorectal cancer cell lines. I had used different matrixes previously from different companies but I should say from my experience that “SpheroSeev” works better than regular spheroid matrix. I like it!

B.N.Nishanth, Ph.D
Lab of Prof. Yosef Yarden
Dept. of Biological Regulation
Weizmann Institute of Science