Every year more and more scientists are finding out that RPA really works. Over 170 peer-reviewed publications*have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren't possible with PCR. *TwistDx takes no responsibility for the content of the publications or their author/s.
Rapid isothermal point mutation detection – towards a first pass screening strategy for multidrug resistant tuberculosis
Author:Ng BYC, Wee EJH, Woods K, Anderson W, Antaw F, Tsang HZH, West NP, Trau M.
Herein, we describe a novel strategy that enabled exquisite point mutation discrimination with isothermal DNA amplification, using mismatched primers in conjunction with a two-round enrichment process. As a proof of concept, the method was applied to the rapid and specific ...
Rapid and visual detection of Mycobacterium tuberculosis complex using recombinase polymerase amplification combined with lateral flow strips
Author:Ma Q, Liu H, Xiang G, Shan W, Xing W.
In this study, we introduced a rapid and visual detection method of MTBC using recombinase polymerase amplification (RPA) combined with lateral flow (LF) strips. The LF-RPA assay, read results with naked eyes, could detect as few as 5 genome copies ...
Quantification of viable and non-viable Legionella spp. by heterogeneous asymmetric recombinase polymerase amplification (haRPA) on a flow-based chemiluminescence microarray
Author:Kober C, Niessner R, Seidel M.
In this work, it was shown for the first time that the combination of a propidium monoazide (PMA) treatment with haRPA, the so-called viability haRPA, is able to identify viable Legionella on DNA microarrays.
Rapid and sensitive detection of canine distemper virus by real-time reverse transcription recombinase polymerase amplification
Author:Wang J, Wang J, Li R, Liu L, Yuan W.
The RT-RPA assay was performed successfully at 40 °C, and the results were obtained within 3 min–12 min. The assay could detect CDV, but did not show cross-detection of canine parvovirus-2 (CPV-2), canine coronavirus (CCoV), canine parainfluenza virus (CPIV), pseudorabies virus (PRV) or ...
Rapid Detection of Plasmodium knowlesi by Isothermal Recombinase Polymerase Amplification Assay
Author:Lai MY, Ooi CH, Lau YL.
The RPA assay exhibited high sensitivity with limits of detection down to one copy of the plasmid. From the specificity experiments, it was demonstrated that all P. knowlesi samples (N = 45) were positive while other Plasmodium spp. (N = 42) and negative samples (N = ...
Establishment of a Real-time Recombinase Polymerase Amplification Assay for Rapid Detection of African Swine Fever Virus
Author:Li L, Liu F, Fan X, Li J, Zou Y, Liu S, Bao J, Wu X, Wang Z.
In this studythe specific primers and exo probes were designed and synthesised by analysing the conserved region of B646L gene of African swine fever virus（ASFV）. A real-time fluorescence RPA method for detection of ASFV was established.
Establishment of Real-time Fluorescent Recombinase Polymerase Amplification Technology (RPA) for the detection of Seneca Valley Virus
Author:Xiaoxu F, Hardenchu C, Wang Y, Wang J, Liu C, Yutian Y, Zhao Y, Zhang Z, Wu X, Wang Z.
A real-time RPA method was established in this study, based on recombinase polymerase amplification technology (RPA). Assemblies of primers and probes targeting SVV 3D gene were developed and screened, while reaction conditions were optimized before sensitivity, specificity and repeatability of ...