유전자 기반 연구
유전자 기반 연구

Automation and Large Scale Plasmid Purification Kits

What are the expected yields from a Mini, Midi, Maxi, Mega or Giga prep?

The number of bacterial cells and the amount of plasmid DNA per cell can vary greatly between cultures based on many factors (strain of E. coli, plasmid copy number, media type, growth conditions). The maximum yields from a Mini, Midi, Maxi, Mega and Giga prep are 20 µg, 50 µg, 1.5 mg, 5 mg, and 10 mg, respectively. The typical yields from a Mini, Midi, Maxi, Mega and Giga prep are 10 µg, 25 µg, 750 µg, 3.5 mg, and 7 mg, respectively.

What are the advantages of the PhyNexus Mini, Midi, Maxi, Mega, and Giga prep kits?

PhyNexus plasmid purification kits offer full automation that is reliable and requires minimal maintenance. The kits are robust and compatible with a wide range of starting culture volumes.The purified plasmid DNA is transfection quality and can be eluted at high concentrations, eliminating the need for an additional ethanol precipitation step

What are the capabilities of the AutoPlasmid MEA?

The AutoPlasmid MEA is capable of purifying plasmid DNA in the mini or midi scale. The throughput is 48 or 36 samples per run, respectively. The instrument is also capable of running all of PhyNexus’ protein and chromatography PhyTip columns.

How is the AutoPlasmid MMG different from other technologies?

The AutoPlasmid MMG was designed to handle a wide range of starting samples.. Reliability is one of the most important qualities of laboratory equipment, and the AutoPlasmid MMG was built to be robust.  Combining a simple set-up with broad sample input requirements gives the AutoPlasmid MMG a distinct advantage over conventional manual purification kits.

What other consumables or accessories are needed besides the Maxi, Mega and Giga prep kit?

Required:

  • 20 mL transfer pipette tips (P/N DPM 00-10-00)
  • Sample prep conical tubes, pyrogen free, sterile conical tubes (P/N DPM 00-020-00)
  • 200 proof ethanol
  • Deionized water

Recommended:

  • AutoPlasmid MMG post-processing kit (P/N DPM 00-30-00)

 

Does the kit need to be refrigerated?

No the kit does not have to be refrigerated. The kit comes with RNase A and it is recommended to store that at -20 °C.

What is the expiration date on the kit?

The kit is guaranteed for 1 year after manufacturing date.