The detection limit down to five copies was achieved within 10 min, which was lower than that of qPCR (ten copies after 25 cycles within 1.5 h). | Conclusions By contrast with qPCR, the RPA method reported here is sensitive, fast, and reliable far enough to detect and quantify V. cholerae in seafood without a set of expensive equipment. The detection limit of this method was down to five copies, which was achieved within 10 min and had high reproducibil- ity in eight independent assays. Based on lolB, the specificity of RPA was highly conserved within the species V. cholerae. The application of RPA in the food industry was completely demonstrated by the analysis of 45 shrimp samples. In con- clusion, RPA was sensitive and fast detects and even quan- tifies the V. cholerae in seafood. |
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TwistAmp exo kit & exo probe 문의 논문
The detection limit down to five copies was achieved within 10 min, which was lower than that of qPCR (ten copies after 25 cycles within 1.5 h).
Conclusions
By contrast with qPCR, the RPA method reported here is sensitive, fast, and reliable far enough to detect and quantify V. cholerae in seafood without a set of expensive equipment. The detection limit of this method was down to five copies, which was achieved within 10 min and had high reproducibil- ity in eight independent assays. Based on lolB, the specificity of RPA was highly conserved within the species V. cholerae. The application of RPA in the food industry was completely demonstrated by the analysis of 45 shrimp samples. In con- clusion, RPA was sensitive and fast detects and even quan- tifies the V. cholerae in seafood.