It sounds like you want to add a nuclease to the basic kit to run the basic kit like the nfo kit. At this time there is no recommended replacement for the nfo enzyme.
You could use labeled primers and no nfo probe as long as you are 100% sure they will not cause non-specific amplification. If that happens, all of your negative reactions will potentially be false positives when analyzed on a lateral flow strip. Labeling the primers only, if they form a primer dimer, will always come up positive.
Trying to use the exo kit instead of nfo is risky in other ways. The exo in the kit is much more active than nfo and still has the 3’ exonuclease activity, so you run the risk of having exo destroying your amplicon before you have a chance to run a lateral flow strip. One possibility is to heat inactivate the exo reaction as soon as your amplification reaction is complete, to try and limit the damage the exo in the reaction can do. However if this is tried, your amplification product will also be denatured and you would have to allow the products to reanneal before running on the strip. You would have to try this in your system to know if it will work for you.
It sounds like you want to add a nuclease to the basic kit to run the basic kit like the nfo kit. At this time there is no recommended replacement for the nfo enzyme.
You could use labeled primers and no nfo probe as long as you are 100% sure they will not cause non-specific amplification. If that happens, all of your negative reactions will potentially be false positives when analyzed on a lateral flow strip. Labeling the primers only, if they form a primer dimer, will always come up positive.
Trying to use the exo kit instead of nfo is risky in other ways. The exo in the kit is much more active than nfo and still has the 3’ exonuclease activity, so you run the risk of having exo destroying your amplicon before you have a chance to run a lateral flow strip. One possibility is to heat inactivate the exo reaction as soon as your amplification reaction is complete, to try and limit the damage the exo in the reaction can do. However if this is tried, your amplification product will also be denatured and you would have to allow the products to reanneal before running on the strip. You would have to try this in your system to know if it will work for you.