nQ16-X4 is a 16-well real-time Quantitative PCR system. Based on optical signal acquisition technology, time-resolved signal separation technology and unique temperature control technology. It reached the advanced level in terms of detection sensitivity, multi-color crosstalk correction, temperature uniformity and accuracy. The instrument has 4 detection channels (optionally expandable to 5 channels), compatible with most fluorescent dyes and probes, and easily realizes multiplex PCR applications.
The instrument supports common applications based on real-time PCR detection assay, including qualitative detection, absolute quantification, etc. It is widely used in many fields of basic medical and biological research such as early clinical diagnosis of diseases, detection of pathogen content, detection of tumor-related genes and drug resistance genes, research on gene expression levels in tissues or cells, and detection of transgenic animals and plants.
Features of the Instrument
1. Based on confocal scanning fluorescence detection technology, it can effectively remove the interference of background light and excitation light, obtain fluorescent signals with high signal-to-noise ratio, and save the use of fluorescent probes and dyes.
2. The instrument uses linear fluorescence time-sharing scanning technology to ensure the uniformity of all fluorescence acquisitions, without the need for special reagent correction, and improve the accuracy and uniformity of CT.
3. High-performance, long-life single-color LEDs are used as excitation light sources, without thermal attenuation, and maintenance-free for life.
4. High-performance silicon PMT can obtain high fluorescence signal under weak excitation light and reduce photo bleaching.
5. The 4/5-color and 16-well detection time can be shorten to 2S, which can minimize the difference in PCR extension time of different wells caused by the detection time, and shorten the experiment time at the same time. The experiment with 40 cycles can be completed within 20 minutes.
6. The block is made of alloy with specially designed heat conduction, it can effectively transfer heat while improving the heating and cooling rate and maintaining the best temperature uniformity, the temperature uniformity can be controlled within the range of ±0.15°C.
7. The instrument uses high-efficiency gold-plated Peltier, which can provide heating and cooling operations with maximum efficiency and a heating and cooling rate of up to 10°C/s.
Features of the Instrument
1. Based on confocal scanning fluorescence detection technology, it can effectively remove the interference of background light and excitation light, obtain fluorescent signals with high signal-to-noise ratio, and save the use of fluorescent probes and dyes.
2. The instrument uses linear fluorescence time-sharing scanning technology to ensure the uniformity of all fluorescence acquisitions, without the need for special reagent correction, and improve the accuracy and uniformity of CT.
3. High-performance, long-life single-color LEDs are used as excitation light sources, without thermal attenuation, and maintenance-free for life.
4. High-performance silicon PMT can obtain high fluorescence signal under weak excitation light and reduce photo bleaching.
5. The 4/5-color and 16-well detection time can be shorten to 2S, which can minimize the difference in PCR extension time of different wells caused by the detection time, and shorten the experiment time at the same time. The experiment with 40 cycles can be completed within 20 minutes.
6. The block is made of alloy with specially designed heat conduction, it can effectively transfer heat while improving the heating and cooling rate and maintaining the best temperature uniformity, the temperature uniformity can be controlled within the range of ±0.15°C.
7. The instrument uses high-efficiency gold-plated Peltier, which can provide heating and cooling operations with maximum efficiency and a heating and cooling rate of up to 10°C/s.